Single-Tube Method for Nucleic Acid Extraction, Amplification, Purification, and Sequencing
نویسندگان
چکیده
منابع مشابه
Single-tube method for nucleic acid extraction, amplification, purification, and sequencing.
BACKGROUND The hepatitis C virus (HCV) genotype determines patient prognosis and duration of treatment, but sequencing of the gene is lengthy and labor-intensive. We used a commercially available nucleic acid extraction system to develop a single-tube extraction-to-sequencing (STETS) method for HCV genotyping. METHODS HCV RNA was purified and amplified in tubes coated with a solid-phase matri...
متن کاملSensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis
Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...
متن کاملNext Generation Sequencing of Microbiomes New Solutions for Nucleic Acid Extraction and PCR Amplification
The Human Microbiome Project and other approaches are dedicated to the exploration of the vast complexity of microbial communities living in association with the human body, including the skin, oral cavity, upper respiratory tract, stomach, intestine, and genitourinary tract. The aim of these projects is to understand host-microbe interactions with regard to human health and disease. Next Gener...
متن کاملAdvances in Single-molecule Nucleic Acid Sequencing
The ability to quickly and accurately obtain sequence information from single molecules of DNA and RNA has far-reaching implications for our understanding of biology. In the work presented here, we have made several advances in the area of single-molecule DNA and RNA sequencing. Specifically, in attempting to increase the read length of DNA polymerase, we have assayed several custom synthesized...
متن کاملNucleic Acid Extraction and Sequencing from Low-biomass Synthetic Mars
ANALOG SOILS. A. Mojarro, J. Hachey, R. Bailey, M. Brown, R. Doebler, G. Ruvkun, M. T. Zuber, and C. E. Carr Department of Earth, Atmospheric and Planetary Science, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139; Department of Molecular Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA, 02114; Claremont Biosolutions, 1182 Monte Vista Av...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2004
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2004.035808